Picture Of The Screen?

I want to be able to read all the pixels of the screen in RGB format. I want to do this because I want to see if something is on screen, I could make it do print screen & all but that makes the mouse dissappear suddenly then to re-appear which is un-desirable. I don't mind if I do or don't get the mouse pointer in the picture but don't want it to dissappear, is there a way I can do this? trying to make it so the program could list RGB values of lots of pixels on screen, other methods I used were slow & printscreen is fast, but has the undesirable mouse dissappearing problem.